The European Chemicals Agency (ECHA) has contracted a consortium, including BASF, to study the reliability and relevance of New Approach Methodologies (NAMs) as alternatives to animal testing.
They will also promote the use of these methods in the future. The aim of the contract is to get additional NAMs accepted by regulatory authorities.
It focuses on molecular biological technologies (OMICS and toxicokinetics). This will reduce the number of animal studies for safety assessments of chemicals. The contract will run for six years and has a total value of €4.2 million in ECHA funding.
Research Partners will Support ECHA in Developing Guidelines
The Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM) in Hanover, Germany leads the contract research project. ITEM, Michabo Health Science, based in Coventry, England, and BASF Metabolome Solutions in Berlin, Germany, are coordinating the work. Other partners include the department for experimental toxicology and ecology at BASF in Ludwigshafen, Germany. The University of Birmingham in England, and the biotech companies BioClavis, based in Glasgow, Scotland, and Novogene Europe, based in Cambridge, England, are also partners.
The research partners will support ECHA in developing guidelines. These guidelines can be used to reliably predict the properties of substances. There is not yet enough safety information for these substances. The grouping and read-across approach uses existing safety data from structurally similar substances to make these predictions.
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This approach is already being used to close data gaps in registrations under the EU chemicals regulation REACH. We would like to expand it further, for example, through the use of molecular biological methods. If grouping and read-across is correctly applied, there is no longer a need to test every substance on animals."
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We will be able to further reduce the number of animal studies and the costs for safety assessments,” said toxicologist professor Hennicke Kamp, BASF Metabolome Solutions' managing director.
The research focuses on evaluating the informative value of OMICS technologies in chemical safety assessments. These technologies can be used to study, among other things, the activation of genes (transcriptomics) or entire metabolic processes (metabolomics) in cultured cells or a living organism. With OMICS technologies, researchers can measure numerous different changes in a biological sample to extrapolate whether substances have a potentially hazardous effect. Transcriptomics can, for example, determine how the activities of genes change after exposure to a certain substance. The researchers can then draw conclusions about changes to cells or organs. Metabolomics technologies can be used to study metabolic products in cells, such as amino acids, lipids, or hormones. If these change, the health of an organism can be evaluated, much like a diagnostic blood test at the doctor’s office.
The research team aims to find out under which conditions OMICS technologies can reliably deliver relevant and reproducible findings. They want to use the findings to assess the safety of chemical substances. Regulatory authorities should then be able to consult these findings when assessing the substances. This could also further reduce the number of animal studies needed in the future. The necessary regulatory guidelines also have to be developed.
The project will also evaluate methods to predict the toxicokinetic behavior of substances in an organism, including how they are absorbed, distributed, and excreted, as well as to evaluate the accumulation and degradation of substances. These methods comprise computational modeling approaches, so-called physiologically based kinetic (PBK) models. To facilitate the application and implementation of such PBK modeling data in regulatory toxicology, the consortium will evaluate the applicability of PBK models and their input parameters, in particular, the in silico (in computers) and in vitro (experiments on cells, tissue, or organs and isolated under controlled conditions outside the body) toxicokinetic parameters. A special focus is to better understand the performance and limitations of these methods, the current knowledge, and data gaps.
Source: BASF